Introduction
The ViraTrapTM lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column.
The viruses are first applied to a purification column and then further purified and concentrated through a concentration unit.
Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C
Before Starting
Familiar with each step by reading this menu and prepare all materials for the procedure.
Safety Considerations
The lentiviral infected cell culture and the purified virus can be potential bio-hazardous material and can be infectious to humans and animals. All protocols MUST be performed under at least Bio-Safety level 2 working conditions.
Materials required but not supplied
Standard TC centrifuge
Swing bucket rotor.
0.45 μm filter unit
Rack holder for column
PBS
Kit Contents
| Catalog# | V1170-00 | V1170-01 | V1170-02 |
| Preps | 1 | 10 | 20 |
| LV Columns | 1 | 5 | 10 |
| Press-On Cap | 1 | 10 | 20 |
| *Centrifugal filter | 1 | 5 | 10 |
| 15 mL Conical Tube | 2 | 10 | 20 |
| Buffer P | 10 mL | 50 mL | 100 mL |
| Buffer LS | 5 mL | 25 mL | 50 mL |
| Buffer MS | 5 mL | 25 mL | 50 mL |
| Regeneration Buffer | 5 mL | 30 mL | 50 mL |
* Centrifugal device can be purchased from Biomiga separated.
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