Hantavirus Real Time PCR Detection

The Biomiga Hantavirus RNA Detection Kit (QP2200) is a multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the qualitative detection of Hantavirus RNA in nucleic acid extracts derived from respiratory specimens (nasopharyngeal swabs, bronchoalveolar lavage) and whole blood. The kit is designed to simultaneously detect two independent viral genomic targets: the nucleocapsid protein (N) gene on the S segment and the RNA-dependent RNA polymerase (RdRp) gene on the L segment. Amplification of the N gene is monitored in the ROX channel, while amplification of the RdRp gene is monitored in the FAM channel. Human RNase P is detected in the VIC channel and functions as an internal control to confirm specimen adequacy and the performance of the nucleic acid extraction process.