Regeneration Buffer， 100 x Nuclease, Reaction Buffer，Nuclease, Binding Buffer, 15 mL Collection Tube， Desalting Tube， Press-On Cap， Mini Columns

Introduction

The ViraTrap^TM Adenovirus maxi purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x10¹² viral particles can be purified from cell culture media of 6 to 8 T75 flasks.

Traditionally the recombinant lentivirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.

Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.

Storage and Stability

All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).

Before Starting

Familiar with each step by reading this menu and prepare all materials for the procedure.

Safety Consideration

The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

Materials required but not supplied

• 1. ddH₂O
• 2. PBS
• 3. 0.45 μm and 0.22 μm filters
• 4. Rack holder for columns

Kit Contents

*Desalting tube could be regenarated and applied on one kind of virus. Desalting tube could be purchased from Biomiga.