SC2 Real Time PCR Detection Kit (3-gene)

The SC2 Real-Time PCR Detection Kit is designed for specific and qualitative detection of SARS-CoV-2 RNA in samples such as oropharyngeal swabs, nasopharyngeal swabs or sputum suspected of SARS-CoV-2.

Principle

The primers and probes are selected from the regions of RNase P, ORF1ab ,N gene and E gene. Three primer/probe sets are designed for specific detection of SARS-CoV-2. An additional primer/probe set to detect human RNase P gene (as an internal control) is also included. RNA extracted from respiratory specimens is reverse transcribed and amplified in a Real-Time PCR Thermocycler (such as Applied Biosystems 7500 Real-Time PCR Instrument). The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to target sequence between the forward and the reverse primers. During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.