Adeno-Associated Virus Purification Mini Kit

Regeneration Buffer, 100 x Nuclease, Reaction Buffer, Nuclease, Binding Buffer, 15 mL Collection Tube, Desalting Tube, Press-On Cap, Mini Columns

Introduction

Adeno-assiciated viruses, belong to the replication deficient parvovirus family, are small single-stranded DNA viruses. AAVs are important gene delivery tools, which have been used in gene therapy and RNAi delivery.

Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components.

The CsCl ultracentrifugation procedure is time consuming and limited to the volume of cell lysate to be processed.

The ViraTrapTM AAV mini purification kit is designed for efficient purification of recombinant AAV2 and AAV-DJ from rAAV vector transfected cell line. Up to 1 x 1012 viral particles can be purified from cell lysate of 2 x T75 flasks.

Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.

Before starting

Familiar with each step by reading this menu and prepare all materials for the procedure.

Stability

The Guaranteed shelf life is 12 months from the date of purchase.

Safety considerations
The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals.

All protocols MUST be performed under at least Bio-Safety level 2 working condition.

Materials Required But Not Supplied

1.ddH2O
2.PBS
3.0.45 μm filter unit and 0.22 μm syringe filter
4.Rack holder for column