CJ236 Electro Competent Cells
Electrocompetent cells are prepared to take up foreign DNA upon exposure to a brief high-voltage electrical pulse (electroporation). The electric field transiently permeabilizes the cell membrane, allowing plasmid DNA or other constructs to enter the cell. Compared to chemically competent cells, electrocompetent cells typically yield higher transformation efficiencies, making them suitable for demanding applications such as library construction and low-copy plasmid transformation.
$500.00
CJ236 Electrocompetent Cells are an E. coli host strain prepared for electroporation-based transformation. The CJ236 strain is deficient in dUTPase (dut⁻) and uracil DNA glycosylase (ung⁻), which causes newly synthesized DNA to incorporate deoxyuracil (dU) in place of thymine (T). This uracil-substituted single-stranded DNA (ssDNA) serves as a template for high-efficiency Kunkel-method site-directed mutagenesis.
CJ236 harbors an F-factor with a chloramphenicol resistance marker (CamR), enabling phage infection for M13 ssDNA production. The strain was derived from BW313 by introducing pCJ105, an Fʹ CmR construct. CJ236 electrocompetent cells are suitable for preparing uracil-containing M13 and phagemid templates for the Kunkel mutagenesis workflow.
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