CJ236 Chemically Competent Cells are an E. coli host strain used to generate uracil-containing single-stranded DNA (ssDNA) for Kunkel-method site-directed mutagenesis. In CJ236, a portion of the thymine (T) residues in newly synthesized DNA is replaced with deoxyuracil (dU) due to deficiency in dUTPase (dut⁻) and uracil DNA glycosylase (ung⁻). This uracil-substituted ssDNA serves as a template for mutagenesis with high efficiency.
CJ236 harbors an F-factor carrying a chloramphenicol resistance marker (CamR), enabling phage infection for M13 ssDNA production.
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