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All inclusive plasmid miniprep kit 50 Preps PD1228-01

{tab Description}   This kit is designed to purify plasmid from 1-12 mL of culture for all possible downstream applications.  You can select one of the four protocols listed accordingly. Here are the key points, Standard miniprep (Protocol I, page 2) Highest yield: Up to 120 µg per prep ( P ...Read more
$80.00 each

Product Code: PD1228-01


  • Product Details

Description

 

This kit is designed to purify plasmid from 1-12 mL of culture for all possible downstream applications.  You can select one of the four protocols listed accordingly. Here are the key points,

  • Standard miniprep (Protocol I, page 2)
  • Highest yield: Up to 120 µg per prep ( Protocol II, page 2)
  • Fast processing time: 10 minutes protocol (Protocol III, page 3)
  • Highest purity: Transfection grade ( Protocol IV, page 4)
  • Eco-friendly: No chaotropics ( Protocol  III, page 3)
  • Safe for researchers: No Chaotropics ( Protocol III, page 3)

Options

Mini

(Protocol I)

Mini II

( Protocol II)

Express Mini

(Protocol III)

Endofree Express Mini

(Protocol IV)

Culture volume

1 - 5 mL

5-12 mL

1-2 mL

1-5 mL

Lysate clearance

Centrifugation

Centrifugation

Lysate clearance column

Centrifugation

Elution Volume

50 µL

100 µL

50 µL

50 µL

Yield ( high copy # plasmid)

up to 40 µg

up to 120 µg

up to 30 µg

up to  30 µg

Preparation time

       

Purity

Molecular Biology Grade

Molecular Biology Grade

Molecular Biology Grade

Transfection Grade

Processing

Centrifuge, Vacuum

Centrifuge, Vacuum

Centrifuge, Vacuum

Centrifuge, Vacuum

Application

Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, etc.

Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, etc.

Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, etc.

Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation, and transfection, etc.