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Description
Express Plasmid Midiprep Kit
ezBindTM Columns(2),Filter Syringe (2),2.0 ml Microfuge tube(4),Plastic wrench,Buffer A1,B1,C1,DNA Washing Buffer*,RNase A (20 mg/mL),Elution Buffer,User Manual(1)
Introduction
Key to the kit is our proprietary DNA binding systems that allow the highly efficient binding of DNA to our ezBindTM matrix while proteins and other impurities are removed by Wash Buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.
Unlike other kits in the markets, no chaotropic salts are contained in the buffer of our patented plasmid purification kit. The purified DNA is guanidine/anion exchange resin residues free.
This kit is designed for fast and efficient purification of plasmid DNA from 50 to 80 mL of E. coli culture. The column has a plasmid DNA binding capacity of 1000 µg.
The purified DNA is ready for high performance of downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Important Notes
Plasmid Copy Numbers: The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 2 times. Please contact our customer service for further information and reference Table 1 for the commonly used plasmids,
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