Search Store

Search

Product Categories

Total RNA Purification

No subcategories

In vivo study

No subcategories

Transfection Reagents

No subcategories

DNA Markers

No subcategories

Microbiology

No subcategories

Biomiga Vs. Competitor

No subcategories

Plasmid Miniprep Kit I 250 Preps PD1211-02

{tab Description} Plasmid Miniprep Kit I Buffer A1, B1, N1, KB, DNA Washing Buffer, Elution Buffer, RNase A, ezBind Columns(50), User Manual(1) Introduction Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while prot ...Read more
$230.00 each

Product Code: PD1211-02


  • Product Details
  • Downloads

Description

Plasmid Miniprep Kit I

Buffer A1, B1, N1, KB, DNA Washing Buffer, Elution Buffer, RNase A, ezBind Columns(50), User Manual(1)

Introduction

Key to the kit is our proprietary DNA binding systems that allow the high efficient reversible binding of DNA to the mini column while proteins and other impurities are removed by wash buffer. Nucleic acids are then eluted with sterile water or elution buffer.

This kit is designed for fast and efficient purification of plasmid DNA from 1 to 4 mL of E. coli culture. The mini column has a plasmid DNA binding capacity of 50 µg. The yield from 1 mL culture is typically around 8 to 12 µg. Plasmid Miniprep Kit II (PD1213), with the plasmid DNA binding capacity of 80 µg, is recommended if higher yield (>50 µg) is desired.

The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of robust cells such as HEK293 cells.