ECL Western Blotting Substrate 500ml

Catalog#	EC1808-25	EC1808-100	EC1808-500
Reagent A	25 mL	100 mL	5x100 mL
Reagent B	125 µL	500 µL	5x500 µL
Sufficient membrane	250cm2	1000cm2	5000cm2
Price	$70.00	$260.00	$719.00

Specifications

Notes

Storage: Store reagent A and B at 4°C

Procedure Summary

1. Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T.

2. Prepare 1X ECL Western Blotting Substrate by mix Reagent A and Reagent B at a 1000:5 ratio (i.e., 5mL Reagent A + 25μL Reagent B for 6 × 8 cm2 membrane) and add it to the blot.

3. Incubate Reagent with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic.

4. Expose blot to X-ray film.

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ECL Western Blotting Substrate 500ml EC1808-500

Description

Specifications

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