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2x SBGR qPCR mix (Rox I) 12.5 mL QP1211-02

{tab Description} Chemically Modified Hot Start Compatible with: ABI 7500; 7500 Fast, StepOne, StepOnePlus, Stratagene Mx3000?, Mx3005P? Mx4000? Features : 2x SYBR qPCR Mix (ROX I) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR reagent. It contains HS Hot ...Read more
$450.00 each

Product Code: QP1211-02


  • Product Details

Description

Chemically Modified Hot Start Compatible with: ABI 7500; 7500 Fast, StepOne, StepOnePlus, Stratagene Mx3000?, Mx3005P? Mx4000?

Features :

2x SYBR qPCR Mix (ROX I) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR reagent.


  • It contains HS Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.

  • It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.

  • The chemically modified HS Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.

  • The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.

  • The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.

  • It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

    Suitable Real Time PCR Detection Systems


  • ABI Prism 7500/7500 Fast

  • Strategene MX3000/MX3005P

  • Corbett Rotor Gene 3000

  • Systems need low ROX signals